Sequence modeling using semisynthetic ribonuclease S.
نویسندگان
چکیده
منابع مشابه
Crystalline semisynthetic ribonuclease-S'.
Crystals of solid phase-derived semisynthetic ribonuclease-S' were prepared and compared with those for native ribonuclease-S' and -S. The semisynthetic species used was the noncovalent complex of synthetic fragment-(1-20), corresponding to residues 1 through 20 of bovine pancreatic ribonuclease-A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase, EC 3.1.4.22), and native ribonuclease-S-(21-...
متن کاملStudies on Ribonuclease S
Specific regions of the polypeptide sequence of pancreatic ribonuclease have been altered by chemical modification and by limited proteolytic digestion in attempts to implicate specific covalent portions of the molecule in the structure and stabilization of the active center. Digestion of the native molecule with trypsin at elevated temperatures has been shown to remove portions of the chain wi...
متن کاملRibonuclease S redux.
The S-peptide and S-protein components of bovine pancreatic ribonuclease form a noncovalent complex with restored ribonucleolytic activity. Although this archetypal protein-fragment complementation system has been the object of historic work in protein chemistry, intrinsic limitations compromise its utility. Modern methods are shown to overcome those limitations and enable new applications.
متن کاملOptical activity of cystine-containing proteins. II. Circular dichroism spectra of pancreatic ribonuclease A, ribonuclease S, and ribonuclease S-protein.
The circular dichroism spectra of pancreatic ribonuclease A, RNase S, and RNase S-protein have been measured in the wave length interval 198 to 300 mp under various conditions. The circular dichroism spectra of RNase A and S are very similar, possibly identical, over this entire wave length interval at neutral pH. That of RNase S-protein is substantially different. It is shown that the portion ...
متن کاملStudies on Ribonuclease S. I. Limited Carboxypeptidase Degradation of Ribonuclease S-protein and Ribonuclease S-peptide: Effects of Changes in Primary Structure on Enzymic Activity.
Specific regions of the polypeptide sequence of pancreatic ribonuclease have been altered by chemical modification and by limited proteolytic digestion in attempts to implicate specific covalent portions of the molecule in the structure and stabilization of the active center. Digestion of the native molecule with trypsin at elevated temperatures has been shown to remove portions of the chain wi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34966-4